Fig. 8.
The exponential phase of recovery from inactivation of T-channels over a wide range of membrane potentials.A, Recovery of the inactivated T-channels is assessed by a similar two-pulse protocol to that in Figure 7, but here the duration of recovery gap potential (Vr) is much longer for study of the later phase of recovery. Treatment of the data with the aforementioned smoothing technique is no longer necessary because the fraction of recovered channels here is generally much larger than that obtained with the very shortVr in Figure 7. The lines are monoexponential fits of the form: fraction recovered = 0.47 − 0.47exp(−t/353) (forVr = −100 mV, and tdenotes length of Vr in milliseconds; thehorizontal axis), fraction recovered = 1 − exp(−t/349) (for Vr = −150 mV), and fraction recovered = 1 − exp(−t/340) (for Vr = −200 mV). B, The recovery rates from inactivation are given by reciprocals of the time constants from the monoexponential fits in A. The average results from three to six cells in each different Vr are shown.