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Effect of extracellular calcium deprivation on membrane ruffling induced by ATP or ADP. Cells were stimulated with PBS (A, D), 50 μm ATP (B, E), or 50 μm ADP (C, F) in BSS with (A–C) or without (D–F) calcium. After stimulation, the cells were fixed and stained with Texas Red-conjugated phalloidin. Arrowheads indicate membrane ruffles. Scale bar, 20 μm.
