Fig. 9.

Rac activation induced by ATP and ADP.A, Translocation of Rac. The cells were stimulated with PBS (a–c), 50 μm ATP (d–f), or 50 μm ADP (g–i) for 5 min and stained with Rac antibody and FITC-conjugated anti-mouse IgG (a, d, g) and Texas Red-conjugated phalloidin (b, e, h). Merged photographs show the colocalization of Rac and F-actin in the ruffling region (f, i), as indicated by arrows. Scale bar, 10 μm. B, Pull-down assay of activated Rac. Cells were stimulated with PBS (control), 50 μm ATP, or 50 μm ADP for 1 min, and pull-down assay was performed as described in Materials and Methods. Although the total amount of Rac in the cell lysate was the same for each stimulation, activated Rac was increased in the pull-down samples from ATP- and ADP-stimulated cells.