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S1 nuclease mapping of the transcription start site. Right lane (P), Undigested S1-arg3.1/arc probe. Middle lane (S1), S1 nuclease-digested S1-arg3.1/arc oligonucleotide after hybridization with total RNA prepared from 60 min serum-stimulated NIH3T3 cells. The sequence of M13 on the left served as a length marker.
