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. 2001 Aug 1;21(15):5484–5493. doi: 10.1523/JNEUROSCI.21-15-05484.2001

Fig. 7.

Fig. 7.

Induced levels of arg3.1/arc and c-fos mRNA can be blocked by calcium channel and protein kinase A inhibitors in PC12 cells. PC12 cells were pretreated for 30 min with the indicated inhibitors and subsequently stimulated by the addition of either KCl or forskolin. A,D, Autoradiographs of Northern blots hybridized to probes specific for arg3.1/arc, c-fos, and GAPDH.B, E, Quantification of hybridization signals after forskolin stimulation. C,F, Quantification of hybridization signals after KCl depolarization. A–C, Effect of the calcium channel blocker nifedipine (10 μm) on induced arg3.1/arc and c-fos mRNA levels (n= 2). D–F, Effect of the protein kinase A blocker H-89 (20 μm) on induced arg3.1/arc and c-fos mRNA levels (n = 3). Error bars represent SEM; *p < 0.05 (two-tailed Student's t test); NS, not significant,p > 0.05. The induction of arg3.1/arc mRNA by forskolin and KCl was completely blocked by the PKA inhibitor.