Skip to main content
. 2001 Aug 1;21(15):5559–5567. doi: 10.1523/JNEUROSCI.21-15-05559.2001

Fig. 6.

Fig. 6.

Effect of pH 5.2 acidification on FMRFamide-induced inward current in isolated RPeD1 neurons.A, Response of a RPeD1 neuron to focal application of a 1 sec pulse of saline at pH 5.2 recorded under TEVC (trace labeled pH 5.2; holding potential, −100 mV). The pH 5.2 pulse caused a very weak transient inward current. A record of an FMRFamide-induced inward current (1 sec pulse; pipette concentration, 0.1 mm) in the same neuron is shown for comparison. B, MeanI–V plot for the pH 5.2-induced inward current in four RPeD1 cells recorded under TEVC. C, Series of FMRFamide-induced inward currents (pipette concentration, 0.1 mm; holding potential, −100 mV) recorded in a RPeD1 neuron while bathed in saline buffered at pH 7.9 or pH 5.2. D, Summary of the effects of bath pH on the amplitude of FMRFamide-induced inward currents in RPeD1 neurons. Statistical analysis revealed that the reduction by acidification to pH 5.2 was significant compared with values at pH 6.7, 6.8, and 7.9 [ANOVA:F(5,39) = 8.982, p< 0.001; followed by pairwise comparisons using post hoc Tukey honestly significant difference (HSD) tests giving p values ≤ 0.001]. The differences between all other pairs of pH values were not statistically significant (Tukey HSD tests: p values between 0.08 and 0.99).