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. 2001 Aug 1;21(15):5559–5567. doi: 10.1523/JNEUROSCI.21-15-05559.2001

Fig. 8.

Fig. 8.

Effects of various bath pH values on desensitization in isolated RPeD1 neurons. Isolated RPeD1 neurons were exposed to series of six FMRFamide pulses (pipette concentration, 0.1 mm, 1 sec) at 15 sec intervals, and the induced inward currents were measured under TEVC (holding potential, −100 mV) in bath media buffered at pH 5.2, 6.0, and 6.7 (MES-buffered), 6.8 and 7.9 (HEPES-buffered), and pH 9.0 (Tris-buffered). The amplitude of the sixth response in each series was expressed as a percentage of the first response, and the mean values for each experiment were plotted against the pH. A statistical analysis revealed that acidification of the bath medium to pH 5.2 significantly reduced desensitization of the FMRFamide-induced inward current compared with all other pH values tested (ANOVA: F(5,29) = 8.684,p < 0.001, followed by post hocTukey HSD tests for pairwise comparison; p ≤ 0.01 for all pairs; **p ≤ 0.01, ***p ≤ 0.001). These tests also showed that none of the differences between the desensitization levels at pH 6.0, 6.7, 6.8, 7.9, and 9.0 were significant (Tukey HSD tests; p values > 0.5).