Fig. 1.

Protein synthesis is required for the acquisition of spatial preferences in the Morris water maze. A, Mean escape latencies during the 5 d of acquisition of spatial learning under anisomycin (filled squares) or saline (open squares). Data are presented in blocks of two trials. B, Representative paths during the probe trial shown for mice that received anisomycin or saline during reversal. The path is shown for the mouse from each group closest to the median of time spent searching in the target quadrant, the number of target platform crossings, total path length, percentage of time floating, and percentage of time thigmotactic. C, Preference during a 60 sec probe trial for the training quadrant location (Targ) for mice given anisomycin (shaded bars) or saline (open bars) during training. Time spent in the adjacent left (AL), adjacent right (AR), and opposite (Opp) quadrants also is shown. D, Preference during the probe for the training quadrant location (Targ) compared with the maximum nontarget (MN). The MN was calculated by determining each animal's most preferred nontarget quadrant. Error bars indicate SE.