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. 2001 Jun 1;21(11):3911–3922. doi: 10.1523/JNEUROSCI.21-11-03911.2001

Fig. 2.

Fig. 2.

Distribution of netrin-1 protein in adult rat spinal cord. A, B, Distribution of netrin-1 immunoreactivity in dorsal (A) and ventral (B) C7 adult rat spinal cord is shown. Netrin-1 immunoreactivity was associated with a heterogeneous population of cell bodies throughout the spinal gray matter and a uniform population in the white matter. C, D, F, Fibrous and punctate immunoreactivity was detected in the neuropil (C, D), including immunoreactivity associated with fine processes and with larger neurites that have a beads-on-a-string morphology characteristic of synaptic varicosities (F; cervical nucleus and lateral spinal nucleus at C7). E, In the ventral horn of the gray matter, netrin-immunoreactive cell bodies and processes characteristic of motoneurons were detected. A, B, In the white matter, a uniform population of small cell bodies and their processes were netrin-1 positive. Bright-field optics, visualized with a POD-conjugated secondary antibody and diaminobenzidine substrate, is shown. Objective magnification: A, B, 10×;C–F, 40×. Scale bars: A, B, 100 μm;C–E, 50 μm; F, 30 μm.