Fig. 3.
Levels of G-proteins in the striatum of D1 receptor knock-out mice. A, Western blot analysis of representative striatal homogenates from D1R knock-out (D1R −/−) mice or wild-type controls (D1R +/+), with antibodies that recognize specifically Gαolf, Gαs, Gαi2, and Gβ. B, The optical density of immunoreactive bands was quantified on films and normalized taking as 100% of the mean optical density of controls on each film (see Materials and Methods). Results correspond to the mean ± SEM of data obtained in 6–16 animals studied in three independent experiments. *p < 0.05 and **p< 0.01 by two-tailed Student's t test.C, Northern analysis of RNA isolated from the striata of D1R knock-out (D1R −/−) mice and wild-type control (D1R +/+) mice. In each lane, the amounts of32P-labeled Gαolf, Gαs, and GAPDH probes bound to the membranes were measured with an Instant Imager. The levels of Gαolf and Gαs mRNA were normalized to those of GAPDH mRNA and were expressed as a percentage of mean value measured in wild-type controls (n = 14–16).