Fig. 3.

BS produces NK₁ receptor internalization in the dorsal horn. Confocal images of the contralateral (A; 10 optical sections) and the stimulated (B; 3 optical sections) side of a representative slice. Insets, Higher magnification of the neurons in the rectangles (8 optical sections inA; 5 optical sections in B). Scale bar: 50 and 16 μm for low- and high-magnification, respectively.I, II, and IIIindicate the location of Rexed's laminas. C, Percentage of NK₁ receptor-immunoreactive (NK₁R-ir) neuronal somata in laminas I-II_o with internalization after BS; mean ± SEM of four slices obtained from two rats. ***p < 0.0001 (t test).