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. 2001 Jun 15;21(12):4125–4133. doi: 10.1523/JNEUROSCI.21-12-04125.2001

Fig. 3.

Fig. 3.

α7 couples Aβ42 activation of ERK2 MAPK in cultured rat hippocampal slices. a, Quantitative immunoblot demonstrates that ERK2 MAPK activation by 100 nm Aβ42 is blocked by 1 μm MLA and 100 μm BTX. Basal, 1.00 ± 0.04; Aβ42 (light-shaded bar), 1.58 ± 0.09; MLA + Aβ42, 1.19 ± 0.07; Aβ42 (dark-shaded bar), 1.67 ± 0.11; BTX + Aβ42, 0.94 ± 0.14. *Significant difference from basal ERK2 MAPK activity; p < 0.0001, Student'st test with Welch's correction because variances differ significantly according to Bartlett's test. Representative immunoblot results are depicted below the response histogram.b, Aβ42 (100 nm) desensitizes the nicotine-induced (500 μm) activation of ERK2 MAPK. Basal, 1.05 ± 0.14; nicotine (nic), 1.94 ± 0.22; Aβ42 (at 2 hr), 1.14 ± 0.24; Aβ42 + nicotine (at 2 hr), 1.03 ± 0.13. *Significant difference from basal ERK2 MAPK activity; p < 0.001, post hoc Tukey multiple comparison test. Representative immunoblot results are depicted below the response histogram. c, Aβ42 (100 nm) does not desensitize the PDA-induced (10 μm) activation of ERK2 MAPK. Basal, 1.00 ± 0.08; Aβ42 (at 2 hr), 0.78 ± 0.11; Aβ42 + PDA (at 2 hr), 2.15 ± 0.54; PDA, 2.25 ± 0.13. *Significant difference from basal ERK2 MAPK activity; p < 0.01, post hoc Tukey multiple comparison test. Representative immunoblot results are depicted below the response histogram.d, The 100 nm Aβ42 activation of ERK2 MAPK is not blocked by TTX and exhibits Ca2+ dependency. Basal, 1.00 ± 0.06; Aβ42, 1.30 ± 0.07; TTX, 0.67 ± 0.10; Aβ42 + TTX, 1.02 ± 0.05; EGTA, 0.59 ± 0.04; Aβ42 + EGTA, 0.65 ± 0.05. *Significant difference from basal-induced ERK2 MAPK activity. #, Significant difference from TTX-induced ERK2 MAPK activity; p < 0.001, post hocTukey multiple comparison test.