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. 2001 Nov 1;21(21):8548–8563. doi: 10.1523/JNEUROSCI.21-21-08548.2001

Fig. 2.

Fig. 2.

Development of topographic projection from peripheral temporal retina to anterior tectum: quantitation of RGC axon overshoot and branch distribution. Axons were labeled by a small focal injection of DiI into peripheral temporal retina, as in Figure 1. The anterior (A)–posterior (P) tectal axis was divided into 500 μm bins; the number of labeled axons and branches in each bin was counted, and the total number at each age was summed. The x-axis plots the location of each 500 μm bin relative to the location of the topographically correct termination zone (TZ) along the A-P tectal axis, which on average was 1 mm from the anterior edge of the tectum.A, Quantitation of RGC axon overshoot. Graphed are the percentages of labeled axons that extend posteriorly past a given point along the A-P tectal axis. B, Distribution of interstitial branches along the axon shaft expressed in percentage. The number of branches in each bin is graphed as the percentage of total branches at a given age. C, Distribution of interstitial branches along the axon shaft expressed as branch density. For each age, the total number of branches in each 500 μm bin was divided by total number of labeled axons within that bin to determine the number of labeled branches per labeled axon per bin. Number of tectal whole mounts: E10 (10), E11 (7), E12 (9), E13 (7). Number of axons: E10 (104), E11 (64), E12 (68), E13 (52). Number of branches: E10 (302), E11(248), E12 (241), E13 (141). See Results for statistical tests.