Fig. 4.
Conditional accumulation of LEO isoforms in the brain of transgenic animals. Paraffin frontal head sections (5 μm) from animals with the genotypes detailed below were challenged with the α-LEO antibody, and the results were visualized by the HRP activity conjugated to the secondary antibody. Control and mutant animals were mounted on the same slide, and slides with all genotypes were processed in parallel. Each genotype was represented by a minimum of nine individuals, and the entire experimental set was repeated three times.I, LEO accumulation in the mushroom body neurons (shown at the level of the lobes). Large arrowhead, The β, β′, γ complex. Small arrowhead, The α, α′complex. Without induction before immunohistochemistry (−HS): A, control animals;B, leo2.3;LI reared at 18°C; C, leoP1375;LI reared under protocol HS B; D,leoP1188/leoP2335;LI reared under protocol HS B; E,leo2.3;LII reared at 18°C;F, leoP1375;LII reared under protocol HS B; G,leoP1188/leoP2335;LI/LII reared under protocol HS B. After two 30 min inductions before immunohistochemistry (+HS): all animals were raised as indicated for the respective genotypes above. Two days after eclosion, the animals were given two 30 min inductions in the cycling incubators delivered 6 hr apart, were allowed a 5–6 hr rest period, and were fixed and processed for immunohistochemistry. H, Control animals; I, leo2.3;LI;J, leoP1375;LI;K,leoP1188/leoP2335;LI;L, leo2.3;LII;M, leoP1375;LII;N,leoP1188/leoP2335;LI/LII.II, LEO accumulation in the ellipsoid body neurons.Arrow, Ellipsoid body. Arrowhead, Mushroom body neuron axonal projections (pedunculi). Animals were raised and treated as indicated above. Without induction:A, control animals; B,leoP1375;LI; C,leoP1188/leoP2335;LI;D,leoP1375;LII; E,leoP1188/leoP2335;LI/LII. After two 30 min inductions: F, control animals;G, leoP1375;LI;H,leoP1188/leoP2335;LI;I,leoP1375;LII; J,leoP1188/leoP2335;LI/LII.