Table 1.
Catalytic activities and lipophilicities of salen manganese complexes
| Compound | SOD activity IC50 (μm) | Catalase rate | Octanol partitioning |
|---|---|---|---|
| EUK-8 | 1.5 ± 0.4 | 103 ± 9 | 8.2 ± 1.8 |
| EUK-134 | 1.3 ± 0.1 | 209 ± 23 | 7.6 ± 0.7 |
| EUK-189 | 1.4 ± 0.5 | 180 ± 9 | 14.2 ± 0.4 |
SOD activity was assayed as described previously (Baker et al., 1998) using oxidized cytochrome c as the electron acceptor. IC50 values (μm) represent the concentration of compound having 1 U of SOD activity. Each value shown is the mean ± SD for IC50 values obtained from eight separate concentration curves, each including seven different concentrations of compound. Catalase activity was assayed by monitoring conversion of hydrogen peroxide to oxygen as described previously (Baker et al., 1998). Initial rate (μmoxygen produced/min) values are the means ± SD for five reactions. Lipophilicity was assessed by solvent partitioning as described in Materials and Methods and is expressed as the percentage of partitioning into octanol (mean ± SD for five samples).