Fig. 6.

Role of FcR in microglial activation. Mouse microglia were purified from the brains of 4- to 5-d-old mice with intact FcR γ chain (FcγR+/+) or with deleted FcR γ chain (FcγR−/−). The microglia were incubated with LPS (4 μg/ml), high-dose PD IgG (200 μg/ml), low-dose PD IgG (20 μg/ml; n = 3), high-dose DA-Q-M membranes (150 μg/ml), low dose DA-Q-M membranes (15 μg/ml), low-dose PD IgG + dose DA-Q-M membranes, and high-dose Fab fragment of PD IgG (200 μg/ml; n = 3) for 2 d. Microglial activation was determined by TNF-α release in the culture medium. *p < 0.005 and **p< 0.001 versus control FcγR+/+ microglia.