Table 1.
Protective effects of catalase, SOD, GSH, NO inhibitors, FcγR−/−, TNF-α, and IL-1β neutralized antibodies on microglia-induced MES 23.5 cell injury
| Treatment | % Protection | |||
|---|---|---|---|---|
| LPS | PD IgG | DA-Q-M | DA-Q-M + PD IgG | |
| Catalase | 90 ± 15.11-165 | 85 ± 18.41-165 | 88 ± 12.21-165 | 81 ± 7.51-165 |
| GSH | 62 ± 9.11-160 | 51 ± 4.21-160 | 65 ± 111-160 | 59 ± 6.41-160 |
| SOD | 10 ± 3.0 | 7 ± 0.8 | 4 ± 1.2 | −2 ± 0.3 |
| L-NIL | 75 ± 6.41-160 | 69 ± 11.01-160 | 58 ± 9.2* | 70 ± 12.51-160 |
| 7-NIO | 8 ± 1.8 | 9 ± 2.2 | 0 ± 0.1 | 11 ± 1.8 |
| GSH + L-NIL | 91 ± 12.21-165 | 89 ± 5.51-165 | 93 ± 121-165 | 84 ± 7.91-165 |
| FcγR−/− | 10 ± 2.9 | 78 ± 18.21-160 | 24 ± 9 | 70 ± 8.21-160 |
| TNF-α antibody | 3 ± 0.6 | −5 ± 0.8 | −2 ± 0.4 | 3 ± 0.4 |
| IL-1β antibody | −4 ± 1.7 | 2 ± 0.5 | −8 ± 1.0 | 4 ± 0.5 |
| TNF-α + IL-1β antibodies | 5 ± 1.2 | 8 ± 1.4 | 6 ± 2.2 | 2 ± 0.5 |
Catalase 100 U/ml, GSH 50 μm, SOD 200 U/ml, iNOS inhibitor L-NIL 100 μm, nNOS inhibitor 7-NIO 100 μm, and neutralized antibodies to TNF-α (50 μg/ml) and to IL-1β (10 μg/ml) were incubated in the cocultures of MES 23.5 cells and microglia 20 min before LPS, PD IgG, and DA-Q-modified MES 23.5 cell membranes. Cell injury was determined by measuring TH activity in MES 23.5 cells cocultured with reactive microglia. Protection was estimated as percentage of inhibition of cell injury in cocultures pretreated with different protective agents versus cocultures treated with 4 μg/ml LPS, high-dose PD IgG (200 μg/ml), high-dose DA-Q-M membranes (150 μg/ml), and low-dose DA-Q-M membranes (15 μg/ml) + low-dose PD IgG (20 μg/ml). Values represent mean ± SEM of triplicate determinations made in three separate experiments.
p < 0.05,
F1-160: p < 0.01, and
F1-165: p < 0.005 versus cultures treated with LPS, PD IgG, DA-Q-M, or DA-Q-M + PD IgG alone.