Fig. 2.
Neural elements that were quantified included circular IMAs, IGLEs, and myenteric neurons. Top, A darkfield photomicrograph from the forestomach of a wild-type mouse. Three parallel telodendria (oriented horizontally; two of the parallel telodendria are identified by arrows) are connected by crossbridges to form one IMA (labeled with WGA-HRP) within the circular muscle layer. A small bundle of labeled sensory axons (oriented diagonally) is present in the top right portion of the image. Middle, A darkfield photomicrograph of an IGLE from the duodenum of a wild-type mouse labeled with WGA-HRP. The IGLE extends horizontally from a small sensory axon bundle that is oriented vertically at the left of the image.Bottom, A brightfield photomicrograph of a myenteric ganglion from the duodenum. Myenteric neurons in whole-mounted GI tract regions were stained with cuprolinic blue. Scale bars, 50 μm.