Fig. 7.

The Cdk inhibitor olomoucine protects primary neuronal cultures subjected to OGD. A, Quantitative assessment of neuronal injury by measurement of LDH release 24 hr after OGD presented as the difference between control cultures and cultures subjected to OGD. Olomoucine treatment produced significant protection at 10 and 100 μm, whereas higher concentrations were toxic to the cultures (data not shown). The results are presented as the mean value ± SEM from three independent experiments performed in triplicate. *p < 0.05 and **p < 0.01 versus vehicle-pretreated sister cultures exposed to OGD; one-way ANOVA followed by Tukey's post hoc test. B, Phase-contrast micrograph of primary cortical neurons in culture. C, Twenty-four hours after 90 min OGD and pretreatment with vehicle (0.02% DMSO).D, Cultures exposed to the same insult as inC but pretreated with olomoucine (10 μm). Scale bar, 70 μm.