Fig. 11.

FGF2 induces TNFα-mediated apoptosis in primary hippocampal cells. Primary embryonic rat hippocampal cells were isolated and expanded as described in Materials and Methods. After 1 d in defined medium, some cultures were treated with FGF2 (10 ng/ml). Cultures were treated with antiserum to TNFα (αTNFα; 5 μg/ml) as indicated.A, Apoptotic cells were counted 2 d after the addition of FGF2 (for 2 d of antibody treatment,p = 0.005; for 1 d of antibody treatment,p = 0.001). B, Cell survival was determined for the same conditions (p = 0.001 for 1 d or 2 d of antibody treatment). These data are representative of three independent experiments.