FLAME-enhanced H19-7 survival in N2 and FGF2-treated cultures. The survival of a population of H19-7 cells stably expressing ectopic FLAME was compared with that of cells stably expressing a control vector in both N2 (A, C, D) and FGF2-treated (B, E, F) conditions. A, B, Data that are representative of two independent experiments in which FLAME cells exhibited better survival than did control cells on day 2 in FGF2 (p = 0.005), on day 3 in both N2 (p = 0.05) and FGF2 (p = 0.005), and on day 7 in both N2 (p = 0.001) and FGF2 (p = 0.001). Representative fields were photographed (400×) on day 7. C, FLAME cells in N2.D, Control cells in N2. E, FGF2-treated FLAME cells. F, FGF2-treated control cells. Scale bar, 20 μm.