Fig. 3.

Dvl-1 has no effect on the rate of turnover of halo-APP. Cells stably expressing APPwt with and without transient dvl-1 transfection were compared using metabolic labeling and pulse chase. Cells were incubated in methionine-free medium followed by incubation in ³⁵S-methionine-containing medium. At baseline and at 2 and 4 hr after the pulse, APP was immunoprecipitated from lysed cells using 22C11 and halo-APP quantitated by autoradiography and densitometry. No significant differences at any time point were observed (n = 3), demonstrating that dvl-1 does not affect turnover of total APP.