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. 2001 Jul 15;21(14):4949–4957. doi: 10.1523/JNEUROSCI.21-14-04949.2001

Fig. 3.

Fig. 3.

MLK3 induces neuronal apoptosis. A, Induction of neuronal cell death by MLK3 in SCG neurons. Rat sympathetic neurons, cultured for 5–7 d in the presence of NGF, were microinjected with 0.3 mg/ml empty expression vector (E) or the different MLK3 constructs [WT, KD, CRIB(−), andKDCRIB(−)] along with 70 kDa Texas Red-dextran to mark the injected cells. Then 48 hr later the percentage of surviving cells was assessed. In each experiment 200 cells were injected. The results are the mean of three independent experiments ± SEM. B, MLK3 increases the number of pyknotic nuclei in SCG neurons. SCG neurons were injected with 0.3 mg/ml control empty expression vector or the various constructs of MLK3. After 24 hr the nuclear morphology was visualized by Hoechst staining. The results are the mean of three independent experiments ± SEM. C, zVAD-fmk protects SCG neurons from MLK3-induced death. The 5- to 7-d-old sympathetic neurons were pretreated with 100 μm zVAD-fmk for 2 hr or were left untreated before microinjection with 0.3 mg/ml WT MLK3. The percentage of surviving cells was assessed 48 hr later. The results are the mean of three independent experiments ± SEM.