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. 2001 Oct 1;21(19):7654–7663. doi: 10.1523/JNEUROSCI.21-19-07654.2001

Fig. 10.

Fig. 10.

In the presence of ACM, Slit repels migrating neurons from SVZ explants without reducing cell number.A, Explants were cultured in Matrigel in the proximity of aggregates of x-Slit-expressing cells (left), Matrigel with ACM (middle), or both (right). The dashed lines indicate the location of the aggregates relative to the explants. Migration was asymmetric in the presence of x-Slit-expressing cells. When Slit was presented alone, very few cell migrated, but robust migration away from the Slit source was observed when ACM was also present.B, Quantification of migration from explants cocultured near x-Slit-expressing aggregates (Slit Agg), with ACM, or both (Slit Agg + ACM). The number of cells migrating from the proximal and distal sides of explants plated with Slit aggregates alone were significantly less than those from explants plated with Slit plus ACM. Moreover, the total number of cells migrating in ACM alone or Slit plus ACM was the same, but their distribution was different. Thus, in the presence of ACM, Slit changes the direction of migration without affecting the number of migrating cells.