Fig. 7.

Aβ induces apoptosis by a Fas ligand–Fas-dependent mechanism. A, B, Cortical neurons were pretreated with 15 μg/ml Fas–Fc or control IgG for 2 hr, and then 25 μm Aβ25–35 was added, and apoptosis was determined by scoring cells with condensed and fragmented nuclei (A) or using the MTS assay (B). *p < 0.05, significantly different from IgG (ANOVA and Dunnett's test or Student's t test). C–E, Cortical neurons were incubated with 2.5 μg/ml anti-Fas antibody or control IgG for 2 hr and then treated with 25 μm Aβ25–35 (C), 200 μm kainic acid (D), or 5 mm KCN (E). Cell death was assessed by the MTS assay. *p < 0.05, significantly different from IgG (Student's t test).