Fig. 5.

TBI-induced specific cleavage of procaspase-3 in rat brain cortex. Eighty microgram aliquots of cytosolic protein extracts isolated from sham control or traumatized rat cortex at indicated times after TBI were subjected to 12% SDS-PAGE and transferred to a nitrocellulose filter. The filter was probed with a rabbit polyclonal antibody against a p17 cleaved form of procaspase-3 (Cell Signaling Technology). To control protein loading, membranes were stripped and reprobed with an antibody against β-actin. A significant increase in caspase-3 cleavage was observed 48 hr after injury.