Fig. 5.

Selective blockade of synaptic input mimics TeTxLC expression. A,Cha^ts2embryos raised at 29°C result in a paralytic phenotype caused by a significant reduction of ACh. At this temperature, aCC/RP2 show no synaptic input (Fig. 1C,D), although their capability to release neurotransmitter is not affected. Under these conditions, aCC/RP2 show increased current densities of bothI_Kfast (shal +I_CF) (Ai) andI_Na (Aii) compared with controls (Cha^ts2/+). Flies were allowed to lay eggs at 18°C for 2 hr periods after which embryos were raised at 29°C. B, At a continuous developmental temperature of 18°C, Cha^ts2 embryos are viable and recordings show that aCC/RP2 receive normal levels of synaptic input (Fig. 1C,D). In addition,I_Kfast (Bi) andI_Na (Bii) are not increased above controls (Cha^ts2/+). Values shown represent mean ± SE; n ≥ 8. Values forI_Kfast and I_Na at 29°C are significantly different from controls (p ≤ 0.01).