Fig. 6.
Alterations in electrical properties produced by the removal of synaptic input are reversible. Ai, By shifting Chats2 embryos from 18°C (permissive temperature) to 29°C (restrictive temperature,black bar) between 13 and 19 hr AEL, it is possible to suppress synaptic input to aCC/RP2 during the period in which electrogenesis occurs in these neurons. After this treatment embryos were returned to 18°C. Recordings at 20 hr AEL show that peakIKfast (shal +ICF) density is significantly increased (p ≤ 0.05). Aii,This temperature shift, however, also delays hatching until ∼23–24 hr AEL. Recordings from aCC/RP2 in embryos on hatching show that peakIKfast density has decreased to control levels. Values shown represent mean ± SE; n≥ 8. All timings shown are normalized to development at 25°C (21 hr development time, 41 hr at 18°C).