Fig. 2.

L1 wild-type and mutant constructs are expressed at equivalent levels in HEK 293 cells. HEK 293 cells were lysed, and aliquots were analyzed by SDS-PAGE and Western blotting with monoclonal antibodies specific for L1 to determine the relative levels of L1 expression. HEK 293 cells were transfected with the ankyrin–GFP expression plasmid and with the following human L1 expression plasmids (L1): none (—); wild-type L1 with or without the RSLE exon sequence (WT+RSLE and WT-RSLE); or the point mutants L1+S1194L (S1194L), L1+S1224L (S1224L), and L1+Y1229H (Y1229H), as indicated. The position of the 175 kDa molecular mass marker is indicated to the left of the panel.