Fig. 7.

Localization of cytoplasmic wild-type and mutant L1 in B35 neuroblastoma cell lines. B35 cell lines stably expressing wild-type L1 (WT+RSLE, A, A′) or the L1 point mutantsS1194L (B, B′), S1224L(C, C′), or Y1229H (D, D′) were fixed and permeabilized before treatment with Neuro4 monoclonal antibodies and were processed for immunofluorescence staining and confocal microscopy as described in Materials and Methods. Theleft column presents confocal microscopy images of immunostained L1 (L1, A–D), and the right column presents differential interference contrast images (DIC, A′–D′). Each of the horizontal panels (A, A′ through D, D′) presents the same field of cells. Scale bar, 10 μm.