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. 2001 Mar 1;21(5):1590–1599. doi: 10.1523/JNEUROSCI.21-05-01590.2001

Fig. 8.

Fig. 8.

KOR-luciferase reporter activities in P19 cells.A, P19 cells, as either control culture (ctrl) or RA-induced cultures (RA), were transfected with k45 or k19 as described in Materials and Methods, and the specific reporter activity (RUL) was obtained by normalizing luciferase units to internal control lacZ units. Similar results were obtained when the luciferase units were normalized to the amount of protein used in the assays (data not shown). *p < 0.025 versus control; **p > 0.025, no significant difference versus control. B, P19 was transfected with k45 and treated with RA, RA plus TSA, or TSA alone. Specific reporter activity was determined as described above. Triplicate cultures were used in each experiment, and two independent experiments were conducted to obtain these results. *p < 0.025 versus control; **p > 0.025, no significant difference versus control; +p < 0.025 versus RA.