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RT-PCR analysis of LIF mRNA in optic nerve and retina. Total RNA was extracted from E17 retinas, purified E17 APCs, and from VECs and pial cells isolated from P1 optic nerves. Subsequently, total RNA was subjected to RT-PCR using LIF-specific primers. RT-PCR or PCR without reverse transcription using GAPDH primers were used as controls. The amplified products were separated on a 1.5% agarose gel and visualized by ethidium bromide.
