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Southern blot hybridization of the RT-PCR amplification of the TGFα and EGFR transcripts from E15 purified motoneuron total RNA. The hybridization probes corresponded to the rat TGFα or the EGFR cDNA probes. −RT, The reverse transcriptase solution was omitted from the PCR reaction. +RT, PCR reaction in the presence of the reverse transcriptase solution.
