Fig. 10.

Expression of other caspases in CNS tissue. CNS tissue obtained from embryos of the following genotypes, wild type, caspase 3−/−, Rb−/−, and Rb/caspase 3 double null, was extracted from forebrain (For.) and hindbrain (Hind.) and separated by SDS-PAGE. Protein was transferred to nitrocellulose and probed with antibodies directed against caspase 2 (Pro-Casp2), 6 (Pro-Casp6), and 7 (Pro-Casp7) as well as actin. A competing peptide was used to confirm specificity of caspase 2 bands and its cleavage product.