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Western blotting indicates the tissue specificity of the antibody to P2X7 receptors. Western blotting of rat brainstem resulted in staining of a major band running at ∼69 kDa when detected with the anti-P2X7 receptor antibody. This is the predicted molecular weight of the P2X7 receptor. Preabsorption of the antiserum with the cognate peptide abolished staining of the membrane.
