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Effects of Hcrt2-SAP on Hcrt receptor mRNA-containing neurons in the TMN. Virtually all of the TMN neurons contain adenosine deaminase (A, B,left side). Hcrt receptor mRNA is present in these neurons (C), and Hcrt fibers and terminals innervate this nucleus (D). A unilateral administration of Hcrt2-SAP to the TMN eliminated these neurons (A, B, right side). Scale bars are in micrometers.
