Fig. 5.

Sensitivity of nicotinic receptor-mediated currents in T stellate cells to cholinergic blockers. Carbachol (500 μm) applied with pressure pulses of 1 sec (bar) evoked biphasic inward currents. A,d-TC (50 μm) blocked both fast and slow components completely and reversibly. In this cell, the fast component was small (arrow). B, MEC (2 μm) blocked both fast and slow components completely and reversibly. C, MLA (20 nm) blocked only the fast component completely and reversibly but did not affect the slow component. D, α-BTX (100 μm) blocked only the fast component completely and irreversibly but did not affect the slow component. E, Strychnine (1 μm) reduced the fast component reversibly by 32%. Cells were held at −60 mV. The internal solution was normal; the external solution contained 2 μm atropine, 0.5 μmTTX, and 40 μm DNQX.