Fig. 9.

Transactivation of the human Phox2a promoter by Phox2b. Luciferase assays were performed to measure the activity of the Phox2a promoter reporter constructs upon their cotransfection in HeLa cells with the pCDNA3 expression vector harboring the Phox2b cDNA or with the empty vector. The bars represent the transcriptional activity of the constructs (indicated on theleft) given as a percentage of the activity of thePstI/NcoI construct when cotransfected with the empty pCDNA3. The data represent the means ± SE (error bars) of at least three independent experiments performed in duplicate. α5 indicates the −1011/+180 construct described inFlora et al. (2000), which corresponds to part of the promoter region of the human α5 nicotinic subunit gene.