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. 2001 Dec 15;21(24):9572–9584. doi: 10.1523/JNEUROSCI.21-24-09572.2001

Fig. 4.

Fig. 4.

Agrin expression in adult XenopusSchwann cells in vitro. A, Agrin isoform expression in cultured Schwann cells was examined by RT-PCR.Lane 1, As a positive control, spinal neurons in culture showed all four agrin isoforms. Lane 2, Muscle in culture displayed only the B0 isoform. Lane 3, Schwann cells in culture expressed B0, B11, and B19 isoforms. Lane 4, SC+M for 7 d also showed B0, B11, and B19 isoforms.Lane 5, As a negative control, the lane showed no bands when no RNA samples were used for RT-PCR. B, Agrin protein in cultured Schwann cells and conditioned media was detected by Western blot using the anti-agrin antibody C3. Lane 1, Native agrin protein over 200 kDa was detected in the total cell lysate of cultured Schwann cells. Lane 2, As a positive control, brain tissues showed similar immunoreactivity over 200 kDa. Conditioned media of Xenopus Schwann cells cultured for 6 weeks (SC-CM; lane 3) andXenopus muscle conditioned media from day 7 in culture (Mu-CM; lane 4) showed a positive band at ∼70 kDa (arrow), but bands over 200 kDa were absent. The 70 kDa band may be an inactive degradation product of native agrin protein.