Fig. 2.

Transfection of hippocampal cells in culture with reporter GFP constructs. A, Schematic of α-CaMKII mRNA and the GFP constructs used for transfections. GFP constructs were modified to contain the last ∼160 nucleotides of α-CaMKII 3′-UTR with either intact CPE sequences (GFP-CPE^WT;top) or mutated CPEs (GFP-CPE^MUT;bottom). B, Hippocampal neurons grown in culture for 7 d, transfected with GFP-CPE^WT_. This culture was processed for GFP fluorescence 8 hr after transfection. GFP-fluorescing neurons are readily distinguished from non-GFP-fluorescing neurons, and GFP is detected throughout the entire neuron (right). Scale bar, 20 μm.