Table 1.

Hypoxia enhances the numbers of neuron-containing EGF-generated spheres and the number of neurons/sphere

Treatment	Frequency of neuronal spheres		Neuron number
	3 DIV	6 DIV	3 DIV	6 DIV
	% of total spheres		Neurons/sphere
Control	13  ± 2	86  ± 6	1.6  ± 0.1	7.1  ± 0.2
4 hr hypoxia	31  ± 1*	83  ± 9	2.5  ± 0.4	11.5  ± 1.01-160
8 hr hypoxia	42  ± 9*	92  ± 3	2.4  ± 0.6	15.4  ± 1.01-160

Three and 6 DIV after a modest hypoxia, single EGF-generated spheres were plated on coverslips in defined media for 7 DIV. The numbers of neuron-containing spheres and neurons/sphere were counted in three independent cultures.

^*p < 0.05 versus control;

F1-160: p < 0.01 versus control.