Table 2.

Persistent actions of a 24 hr hypoxia or EPO treatment in enhancing neurogenesis after washout suggests a direct action on embryonic neural stem cells

	Hypoxia (hr)		Erythropoietin (IU/ml)
	0	4	0	0.1	1	10
	Neurons (% of total cells)
Control	4.9  ± 0.6	11.3  ± 0.8*	4.3  ± 0.4	3.9  ± 0.4	7.6  ± 0.6*	9.1  ± 0.7*
24 hr wash	4.0  ± 0.6	7.7  ± 0.8*	3.7  ± 0.8	3.0  ± 0.5	6.5  ± 0.5*	8.2  ± 0.5*

Control, hypoxic, or EPO-stimulated neural stem cells were washed after 24 hr and replaced with media containing EGF only. After 7 DIV, resultant spheres were dissociated and plated for a further 7 DIV and then examined for neuron number as a percentage of total cells.

^*p < 0.01 versus control.