Table 3.

Effects of injected anti-NF-M on mitochondrial staining

	n	Mitochondrial clusters per neurite	Intensity (IU) per mitochondrial cluster	Intensity per unit neurite length (IU/μm)	Soma intensity (IU/μm2)
Labeled neurons	23	23.9  ± 2.7	698  ± 92	99.4  ± 7.1	575.2  ± 20.4
Unlabeled neurons	30	24.9  ± 2.4	462  ± 36	64.5  ± 4.5	411.9  ± 20.4
Level of significance		NS (p > 0.5)	p < 0.05	p < 0.0001	p< 0.0001

Embryos were injected with a mixture of rhodamine–dextran and anti-NF-M at the two-cell stage, then cultured and stained at 24 h after plating with 4-Di-2-Asp, to label mitochondria in living cells. Labeled and unlabeled neurons were descended from the injected and uninjected blastomeres, respectively. Data are from neurons with long neurites (>85 μm) from three separate cultures imaged under the same conditions to quantitate the intensity of 4-Di-2-Asp staining.n represents the number of neurites imaged. The intensities were measured as described in Materials and Methods and are given in relative intensity units (IUs) averaged among neurites (mean ± SE). The statistical levels of significance of the differences between labeled and unlabeled cells were determined by two-tailed ttest.