Fig. 1.

Various levels of expression of wild-type and A53T mutant α-synuclein in clonal PC12 cell lines. Various clonal PC12 cell lines were generated expressing empty vector (P1 and P2), wild-type α-synuclein (S4, S10, and S12), or A53T mutant α-synuclein (M1, M13, M15, and M18). Triton-soluble lysates (200 μg of protein) were generated, resolved by 13% SDS-PAGE, and immunoblotted with a monoclonal synuclein antibody (1:1000; Transduction Laboratories). Two separate blots are presented. In the blot on the left, a control lysate from rat cortex (30 μg) was used in the first lane. Theasterisk indicates the broad 18 kDa band seen in the control lysate and in the lines expressing α-synuclein. Note the 45 kDa band (arrow) present in the control lysate, the empty-vector controls, and the overexpressing lines. In the blot on theright, lysates of the M15 and M18 lines were similarly generated and processed. Three more empty vector lines (P3, P4, and P5) were generated and showed a pattern that was similar to that seen for P1 and P2 on immunoblotting (Stefanis et al., 2001).