Absence of depolarization-induced dopamine release from PC12 cells expressing A53T α-synuclein. Cells from theP4, S12 (S12a andS12b, from two separate subclones in two different experiments), M1, M15, andM18 lines were plated on 24-well dishes, and depolarization-induced dopamine release (A) and intracellular dopamine (B) were assessed as described in Materials and Methods. The results are reported as mean ± SD (n = 4).