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The perfusion of tetrodotoxin (TTX; 1 μm) reversed the increase in 5-HT output induced by the local application of DOI 100 μm (n = 4). Bars show the period of drug application. The effect of DOI alone is shown by a dotted line.+p < 0.05 versus baseline (DOI alone); *p < 0.05 versus DOI alone (DOI + drug); Duncan test post-ANOVA.
