Fig. 7.

GST-fused JNK1β1 can precipitate APPs in a JIP-1b-dependent manner. COS cells were transfected with the indicated combinations of wt APP or its empty vector (+ or − in wt APP), T7-JIP-1b or its empty vector (+ or − inJIP-1b), and GST orGST-JNK1β1, as indicated with + under eachlane, and their lysates were precipitated with glutathione beads. The presence of APP, JIP-1b, and GST-fused proteins in lysate (lysate) and precipitants (ppt) was detected with anti-APP, anti-T7, and anti-GST antibodies, respectively. Other designations are the same as in Figure 6. The data in this figure are representative of three independent experiments.