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. 2001 Sep 1;21(17):6687–6693. doi: 10.1523/JNEUROSCI.21-17-06687.2001

Fig. 3.

Fig. 3.

Atm function is distinct between the ventricular and subventricular zones. Comparative analysis of WT andAtm−/− embryos at E12.5 and E15.5 showed a marked reduction in radiation-induced death in theAtm−/− SVZ, as indicated by the asterisks. Regions of proliferation at E12.5 and E15.5 are indicated by Ki67 (a, k) and differentiation at E15.5 by Tuj1 (l) immunostaining.Atm expression at E12.5 is shown by in situ hybridization in b. In the ganglionic eminence and primordial plexiform layer at E12.5 (c–h), no differences in p53 immunostaining between WT andAtm−/− are observed: no irradiation (c, d), irradiated WT (e, f), or irradiatedAtm−/− (g, h). In the WT E15.5 neopallial cortex (m–r) p53 stabilization and serine-18 phosphorylation are widespread (o, p), whereas they are restricted to the ventricular zone inAtm−/− (q, r). Apoptosis is present in WT (i), but not in the ventricular zone of the E12.5p53−/− embryo (j). Regions of caspase-3 activation in E15.5 WT (s) andAtm−/−(t) coincided with p53 stabilization and phosphorylation.