Table 3.

Pitx2 does not activate the mutant unc-25/GAD reporter, and ectopic Pitx2 drives ectopic unc-25/GAD expression

Expression construct	Reporter	GFP expression	Number of worms scored	Number of transgenic lines
Punc-30∷Pitx2	Wild-type P unc-25∷GFP	+	20	4
Punc-30∷Pitx2	Mutant Punc-25∷GFP	−	20	3
Phsp-16-2∷Pitx2	Wild-type Punc-25∷GFP	Ectopic3-150	20	12
Phsp-16-2 vector	Wild-type Punc-25∷GFP	−	20	2
Phsp-16-41∷Pitx2	Wild-type Punc-25∷GFP	Ectopic3-150	20	3
Phsp-16-41 vector	Wild-type Punc-25∷GFP	−	20	2

F3-150: Ectopic expression of Punc-25∷GFP was seen in non-D type neurons, in the gut and pharynx.