Confocal image analysis of MAP segregation in neuronally differentiated P19 cells. a–d, P19 stable cell expressing GFP-tau–cod-H construct, neuronally differentiated for 9 d. MAP segregation was analyzed by confocal microscopy. Scale bar, 5 μm. a, GFP-tau protein is visible (green) in the soma and axon of a single cell.b, MAP-2 is visible (red) in the soma and dendrite of the same cell. c, Computer-merged analysis of GFP-tau (green) and MAP-2 (red) proteins; GFP-tau and MAP-2 colocalize (orange) in the soma but segregate to the axon and the dendrite, respectively.d, Phase view of the same P19 cell. e, The same cell stained with tau-1 antibodies. Large filled arrowheads denote an axon, large open arrowheads denote dendrites, and small filled arrowheads denote a neuronal cell body.